Khloe Kardashian has been 'Punk'd' by Kelly Osbourne and Miley Cyrus.

The 27-year-old star is the focus of a new episode of the comedy show, which is returning without original host Ashton Kutcher, and she is fooled into believing a delivery man has caught his genitals in his trouser zipper while he briefly uses Miley's bathroom.

Speaking in a preview of the episode, Miley said: "Khloe thinks she's going to have girls' hangout time and order food. And when the delivery guy shows up, he's going to ask to use my bathroom. He's going to get his balls zipped into the zipper of his pants. Khloe, you're going to get Punk'd!"

When the fake accident happens, Khloe maintains a level head and quickly calls the emergency services to ask what to do, and is told to quickly elevate him onto a counter.

The actor is then seen stating he needs to stand up, to which she responds: "Sir the paramedic said if you do not lay down you may lose your testicle, please."

The conclusion of the prank is not shown.

Ashton - who last year split from wife of six years Demi Moore - has continued to work on the show as an executive producer and has revived the series by featuring a different celebrity host on every episode.